Genome-wide analysis and expression of the calcium-dependent protein kinase gene family in cucumber
0303 health sciences
Sequence Homology, Amino Acid
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Molecular Sequence Data
Temperature
Genetic Variation
Response Elements
Chromosomes, Plant
03 medical and health sciences
Plant Growth Regulators
Gene Expression Regulation, Plant
Multigene Family
Cluster Analysis
Amino Acid Sequence
Cucumis sativus
Promoter Regions, Genetic
Protein Kinases
Genome, Plant
Phylogeny
Plant Proteins
DOI:
10.1007/s00438-015-1002-1
Publication Date:
2015-02-17T06:15:59Z
AUTHORS (8)
ABSTRACT
Calcium-dependent protein kinases (CDPKs) are multi-functional proteins that combine calcium-binding and signaling capabilities within a single gene product. Current studies have shown that the CDPKs regulate numerous growth and developmental processes and biotic and abiotic stress responses. Nonetheless, knowledge concerning the specific expression patterns and evolutionary history of the CDPK family in cucumber (Cucumis sativus L.) remains very limited. We, therefore, investigated the phylogenetic relationships and expression profiles of the 19 CDPK genes identified in the cucumber genome sequence, resolving them into four subfamilies based on a phylogenetic tree and gene structures. Tissue-specific expression profiles suggest that cucumber CDPK genes are involved in cucumber tissue development. An expression analysis based on qRT-PCR indicated that cucumber CDPK genes are extensively involved in abscisic acid, salt, cold, drought, heat, and waterlogging responses, possibly by different mechanisms. The fates of two paralogs after divergence were also investigated, suggesting subfunctionalization and neofunctionalization during evolution. These observations lay an important foundation for functional and evolutionary analyses of the CDPK gene family in cucurbitaceae species.
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CITATIONS (59)
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