Tenuigenin promotes the osteogenic differentiation of bone mesenchymal stem cells in vitro and in vivo

• DOI: 10.1007/s00441-016-2528-1
• Publication Date: 2016-11-14T00:42:21Z
• Authors: Hua-ji Jiang; Xing-gui Tian; Shou-bin Huang; Guo-rong Chen; Min-jun Huang; Yu-hui Chen; Bin Yan; Sheng-fa Li; Jia-jun Tang; Hui-yu Zhao; Liang Wang; Zhong-min Zhang
• Keywords: 0301 basic medicine; Histology; Ovariectomy; Osteocalcin; Core Binding Factor Alpha 1 Subunit; Models, Biological; Bone and Bones; Pathology and Forensic Medicine; 03 medical and health sciences; Osteogenesis; Animals; Femur; Bone Resorption; Wnt Signaling Pathway; Regular Article; Cell Differentiation; Mesenchymal Stem Cells; Cell Biology; 3. Good health; Mice, Inbred C57BL; Sp7 Transcription Factor; Female; Biomarkers; Drugs, Chinese Herbal; Transcription Factors

Osteoporosis, which is a systemic skeletal disease characterized by low bone mineral density and microarchitectural deterioration of quality, global increasing public health problem. Recent studies have suggested that Tenuigenin (TEN), class native compounds with numerous biological activities such as anti-resorptive properties, exerts protective effects against postmenopausal loss. The present study aims to investigate the osteogenic TEN on mesenchymal stem cells (BMSCs) in vitro vivo. Alkaline phosphatase (ALP) activity/staining, Alizarin red staining expression markers, including runt-related transcription factor 2, osterix, osteocalcin, collagen Iα1, β-catenin glycogen synthase kinase-3β were investigated primary femoral BMSCs from C57/BL6 mice cultured under conditions for 2 weeks examine TEN. An ovariectomized (OVX) mouse model was used effect treatment 3 months We found ALP activity, mineralized nodules markers increased WNT/β-catenin signaling enhanced Bone parameters, trabecular thickness, number higher OVX+TEN group than control OVX mice. Our results suggest therapeutic potential patients osteoporosis.