Macrophage type modulates osteogenic differentiation of adipose tissue MSCs

• DOI: 10.1007/s00441-017-2598-8
• Publication Date: 2017-03-30T00:12:52Z
• Authors: Yang Zhang; Thomas Böse; Ronald E. Unger; John A. Jansen; Charles James Kir...; Jeroen J. J. P. v...
• Keywords: Osteoimmunology; Oncostatin M; Adipose tissue macrophages

Since the reconstruction of large bone defects remains a challenge, knowledge about biology healing is desirable to develop novel strategies for improving treatment defects. In osteoimmunology, macrophages are central component in early stage physiological response after injury and remodeling late stage. During this process, switch macrophage phenotype from pro-inflammatory (M1) anti-inflammatory (M2) observed. An appealing option regeneration would be exploit regulatory role benefit osteogenic differentiation osteoprogenitor cells (e.g., mesenchymal stem cells; MSCs) eventually utilize improve therapeutic outcome regenerative treatment. view this, we focused on vitro interaction different subtypes with adipose tissue MSCs monitor behavior (i.e. proliferation, mineralization) latter dedicated co-culture models. Our data show that M2 macrophages, but not M1 or M0 results significantly increased MSC mineralization caused by soluble factors. Specifically, promoted proliferation MSCs, while solely stimulated middle stages during co-culture. Secretion factors oncostatin M (OSM) morphogenetic protein 2 (BMP-2) showed correlation gene expression levels OSM-receptor BMP-2, suggesting involvement both signaling pathways MSCs.