Developing a 3D intestinal epithelium model for livestock species

• DOI: 10.1007/s00441-018-2924-9
• Publication Date: 2018-09-26T02:29:51Z
• Authors: Hayley Derricott; Lisa Luu; Wai Yee Fong; Catherine S. Hartley; Luke J. Johnston; Stuart D. Armstrong; Nadine Randle; Carrie A. Duckworth; Barry J. Campbell; Jonathan M. Wastling; Janine L. Coombes
• Keywords: Organoid; Intestinal epithelium; Intestinal mucosa; Matrigel

The in vitro 3D culture of intestinal epithelium is a valuable resource the study its function. Organoid exploits stem cells' ability to regenerate and produce differentiated epithelium. Intestinal organoid models from rodent or human tissue are widely available whereas large animal not. Livestock enteric zoonotic diseases elicit significant morbidity mortality populations. Therefore, livestock species-specific may offer novel insights into host-pathogen interactions disease responses. Bovine porcine jejunum were obtained an abattoir their crypts isolated, suspended Matrigel, cultured, cryopreserved resuscitated. 'Rounding' occurred followed by budding then enlargement organoids. Epithelial cells characterised using immunofluorescent staining confocal microscopy. Organoids successfully infected with Toxoplasma gondii Salmonella typhimurium. This model offers long-term, renewable for investigating infections variety pathogens.