Production of carotenoids and lipids by Rhodococcus opacus PD630 in batch and fed-batch culture

• DOI: 10.1007/s00449-016-1681-y
• Publication Date: 2016-09-18T22:48:46Z
• Authors: Anusith Thanapimm...; Tharatron Suwaleerat; Maythee Saisriyoot; Yusuf Chisti; Penjit Srinophakun
• Keywords: Laboratory flask; Sodium acetate; Ammonium sulfate

Production of carotenoids by Rhodococcus opacus PD630 is reported. A modified mineral salt medium formulated with glycerol as an inexpensive carbon source was used for the fermentation. Ammonium acetate was the nitrogen source. A dry cell mass concentration of nearly 5.4 g/L could be produced in shake flasks with a carotenoid concentration of 0.54 mg/L. In batch culture in a 5 L bioreactor, without pH control, the maximum dry biomass concentration was ~30 % lower than in shake flasks and the carotenoids concentration was 0.09 mg/L. Both the biomass concentration and the carotenoids concentration could be raised using a fed-batch operation with a feed mixture of ammonium acetate and acetic acid. With this strategy, the final biomass concentration was 8.2 g/L and the carotenoids concentration was 0.20 mg/L in a 10-day fermentation. A control of pH proved to be unnecessary for maximizing the production of carotenoids in this fermentation.