Near-infrared fluorescence laparoscopy of the cystic duct and cystic artery: first experience with two new preclinical dyes in a pig model

• DOI: 10.1007/s00464-017-5450-z
• Publication Date: 2017-03-07T21:45:17Z
• Authors: Jacqueline van de...; Mahdi Al-Taher; Shu Gi Hsien; Nicole D. Bouvy; Laurents P. S. St...
• Keywords: Cystic artery; Fluorescence-lifetime imaging microscopy; Indocyanine Green

Imaging techniques that enhance visualisation of the anatomy may help prevent bile duct injury. Near-Infrared Fluorescence is such a technique. Previous experiments with ICG have shown illumination extra-hepatic ducts feasible. Yet, there room for improvement in target as compared to background. Experiments IRDye® 800CW show promising results. However, this dye too expensive routine clinical use. The aim study test first applicability two newly developed preclinical dyes regarding intraoperative imaging cystic and artery, 800CW. Laparoscopic cholecystectomy was performed three pigs, using laparoscopic fluorescence system. Each pig received 6 mg one fluorescent (1 mg/mL; 800CW, 800BK or 800NOS) by intravenous injection. Intraoperative recognition biliary system artery registered at set time points. All procedures were digitally recorded, background ratio (TBR) determined assess signal. With all dyes, directly visualised. For duct, 15, 34 30 min needed 800BK, 800NOS respectively. maximum TBR highest (4.20) after 36 min, 2.45 2.15 both 45 min. There no adverse events. seem be good alternatives pigs.