Heat stress inhibits TLR4-NF-κB and TLR4-TBK1 signaling pathways in broilers infected with Salmonella Typhimurium

• DOI: 10.1007/s00484-021-02146-5
• Publication Date: 2021-06-01T13:06:03Z
• Authors: Wei-Hao Li; Yi-Lei Liu; Jian-Chi Lun; Yong-Ming He; Lu-Ping Tang
• Keywords: Salmonella infection; Jejunum; Proinflammatory cytokine

Abstract With the global warming, harm of heat stress (HS) to breeding industry has become more common, which causes decline animal production performance and low immunity. This study aimed analyze effect HS on intestinal immune function Salmonella- infected chickens. Fourteen-day-old broilers were divided into following four groups eight replicates: control (Control), (HS), Salmonella Typhimurium (ST), + (HS+ST). The subjected a 35 °C from 15 28 days age. (ST, 14028, 10 9 cfu/mL) was inoculated, via oral administration at 29 age, ST HS+ST group birds. On 4th day after administration, an increase in jejunum IgA levels observed chickens with Typhimurium. Mechanistic regulation TLR4-NFκB-NLRP3 TLR4-TBK1 signaling by evaluated Typhimurium–infected broilers. Heat markedly inhibited expression cytokines including TNF-α, IL-6, IL-1β, NLRP3, caspase-1, NF-κB-p65, p-NF-κB-p65, IFN-α, IFN-γ, p-IRF3, p-TBK1 Collectively, our results demonstrate that can inhibit response downregulating pathways