Ferrocene-functionalized nanocomposites as signal amplification probes for electrochemical immunoassay of Salmonella typhimurium

• DOI: 10.1007/s00604-020-04579-y
• Publication Date: 2020-10-09T04:03:31Z
• Authors: Sheng-Jun Bu; Kui-Yu Wang; Xiu Liu; Li Ma; Hong-Guo Wei; Wen-Guang Zhang; Wen-Sen Liu; Jia-Yu Wan
• Keywords: Immunoassay; Salmonella typhimurium; Metallocenes; Electrochemical Techniques; Ferrous Compounds; 02 engineering and technology; 0210 nano-technology; Sensitivity and Specificity; 01 natural sciences; Nanocomposites; Signal Transduction; 0104 chemical sciences

An electrochemical immunosensor based on ferrocene (Fc)-functionalized nanocomposites was fabricated as an efficient electroactive signal probe to amplify electrochemical signals for Salmonella typhimurium detection. The electrochemical signal amplification probe was constructed by encapsulating ferrocene into S. typhimurium-specific antimicrobial peptides Magainin I (MI)-Cu3(PO4)2 organic-inorganic nanocomposites (Fc@MI) through a one-step process. Magnetic beads (MBs) coupled with antibody were used as capture ingredient for target magnetic separation, and Fc@MI nanoparticles were used as signal labels in the immunoassays. The sandwich of MBs-target-Fc@MI assay was performed using a screen-printed carbon electrode as transducer surface. The immunosensor platform presents a low limit of detection (LOD) of 3 CFU·mL-1 and a linear range from 10 to 107 CFU·mL-1, with good specificity and precision, and was successfully applied for S. typhimurium detection in milk. Graphical abstract One-pot process antimicrobial peptides Magainin I-Cu3(PO4)2 organic-inorganic nanocomposites (Fc@MI) were used as ideal electrochemical signal label, integrating both essential functions of biological recognition and signal amplification. Screen-printed carbon electrode (SPCE) was used as the electrochemical system for Salmonella typhimurium detection.