A colorimetric sensor for Staphylococcus aureus detection based on controlled click chemical-induced aggregation of gold nanoparticles and immunomagnetic separation

• DOI: 10.1007/s00604-022-05211-x
• Publication Date: 2022-02-14T07:02:57Z
• Authors: Yushen Liu; Xuechen Wang; Xuening Shi; Mengyue Sun; Luliang Wang; Zhenhua Hu; Fangjie Liu; Quanwen Liu; Ping Wang; Juan Li; Chao Zhao
• Keywords: Staphylococcus aureus; Immunomagnetic Separation; Humans; Metal Nanoparticles; Colorimetry; Biosensing Techniques; Gold; 01 natural sciences; 0104 chemical sciences; 3. Good health

Staphylococcus aureus (S. aureus) is a pathogen closely associated with foodborne diseases. We prepared a reliable colorimetric sensor to detect S. aureus using click chemical reaction and immunomagnetic separation. Aptamer-functionalized and ALP-labeled Fe3O4 NPs act as separation and signal transduction elements. Under the optimized conditions, the Cu+ generated by signal transduction triggers a click chemistry reaction, which causes the aggregation of azides and alkyne-AuNPs and a color change. The net extinction ratio of Δ(A530/A760) was linearly correlated with the S. aureus concentration from 10 to 106 cfu mL-1, and the limit detection was 2.4 cfu mL-1. The recoveries were 91.15 ~ 106.36% for the analysis of spiked food and water samples without pre-enrichment. Therefore, we believe that the detection platform can be easily and accurately used for S. aureus detection, providing a broad prospect for on-site visual detection.