SERS biosensor with plastic antibodies for detection of a cancer biomarker protein

• DOI: 10.1007/s00604-024-06327-y
• Publication Date: 2024-04-04T02:01:46Z
• Authors: Daniela Oliveira; Mariana C. C. G. ...; Felismina T. C. M...
• Keywords: Molecularly imprinted polymer; Nanochemistry; Surface-Enhanced Raman Spectroscopy; Sodium dodecyl sulfate

Surface-enhanced Raman scattering (SERS) is a powerful method for detecting breast cancer-specific biomarkers due to its extraordinary enhancement effects obtained by localized surface plasmon resonance (LSPR) in metallic nanostructures at hotspots. In this research, gold nanostars (AuNSs) were used as SERS probes detect cancer biomarker very low concentrations. To end, we combined molecularly imprinted polymers (MIPs) detection layer with the of CA 15-3 point-of-care (PoC) analysis. This required two main steps: (i) deposition MIPs on electrode, followed second step (ii) antibody binding AuNSs containing suitable reporter enhance signaling (SERS). The MPan sensor was prepared electropolymerization monomer aniline presence 15-3. template molecule then extracted from polymer using sodium dodecyl sulfate (SDS). parallel, control material absence protein (NPan). Surface modification performed electrochemical techniques such cyclic voltammetry (CV) and impedance spectroscopy (EIS). performance evaluated technique, which first incubated exposed probe. Under optimized conditions, device showed linear response concentrations 0.016 248.51 U mL