Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density

• DOI: 10.1007/s00701-009-0188-6
• Publication Date: 2009-02-23T09:32:02Z
• Authors: Sven Rainer Kante...; Jan Leppert; Jan Werner Kantel...; Erich Reusche; Gereon Hüttmann; Alf Giese
• Keywords: Two-photon excitation microscopy; Human brain; Brain tumor; Histopathology

Recent studies have highlighted the importance of complete resection a brain tumour but task often remains challenge for neurosurgeon. New technologies which add objective information beyond visualisation provided by traditional operating microscope are required. In this study, we analysed cellular density tumour/brain interface using three dimensional multi-photon microscopy intensity-images experimental gliomas and human brain-tumour biopsy samples. The nuclei was determined in specimens mouse model biopsies analysis optical tissue sections. Three image stacks were compared to serial H&E stained sections conventional histolopathology. Both techniques consistently showed good correlation cell values solid versus adjacent brain. that native on density. Multi-photon is an efficient rapid tool study tissue. allows detection individual cells clusters may therefore provide identification highly lesions during tumours.