Sequence analysis of an Australian isolate of sugarcane bacilliform badnavirus

• DOI: 10.1007/s00705-002-0879-2
• Publication Date: 2003-03-05T11:20:13Z
• Authors: R. J. Geijskes; K. S. Braithwaite; J. L. Dale; R. M. Harding; G. R. Smith
• Keywords: 0303 health sciences; Sequence Homology, Amino Acid; Molecular Sequence Data; Australia; Genetic Variation; Genome, Viral; Sequence Analysis, DNA; 630; Saccharum; 3. Good health; Open Reading Frames; 03 medical and health sciences; Amino Acid Sequence; Cloning, Molecular; Badnavirus; Sequence Alignment; Phylogeny

The genome of an Australian isolate of Sugarcane bacilliform virus (SCBV-IM) was cloned, sequenced and analysed. The genome consisted of 7687 nucleotides and contained three open reading frames which were similar in size and organisation to those of other badnaviruses. SCBV-IM was found to be most similar to the SCBV-Morocco isolate with amino acid sequence similarity of 91.4 %, 83.8 % and 85.3 % in the ORF I, II and III coding regions, respectively. Phylogenetic analysis of the SCBV-IM ORF III deduced amino acid sequence showed that SCBV isolates were more closely related to each other than to other badnaviruses. Amplification of SCBV sequences from three different sugarcane varieties revealed considerable variability in the viral populations, both within single infected plants as well as between infected plants, suggesting that the SCBV isolates sequenced to date may not be representative of the range of virus variability.