Genetic analysis of fish iridoviruses isolated in Taiwan during 2001–2009

• DOI: 10.1007/s00705-011-1017-9
• Publication Date: 2011-05-20T04:31:36Z
• Authors: Sue-Min Huang; Chen Tu; Chun-Hsien Tseng; Chin-Cheng Huang; Chi-Chung Chou; Hung-Chih Kuo; Shao-Kuang Chang
• Keywords: Iridovirus; Ranavirus; Genetic Analysis; Virus classification

To investigate the genetic relationships between field strains of iridoviruses gathered from various fish species in Taiwan, viruses that were collected 2001 to 2009 analyzed. Open reading frames encoding viral major capsid protein (MCP) and adenosine triphosphatase (ATPase) sequenced for phylogenetic analysis. Our results indicated Taiwan aquaculture fishes could be classified into two groups: prior 2005, closely related members genus Ranavirus; after they similar Megalocytivirus. Based on analysis MCP amino acid sequences, virus isolates divided 4 genotypes ISKNV, RSIV, FLIV, GIV, respectively. Pairwise comparisons genes showed ranavirus was an epidemic pathogen economically important production regions cultured marine fish, while megalocytivirus sensitive host range. In addition, distribution synonymous non-synonymous changes gene revealed evolving slowly, most variations mutations. The Ka/Ks values lower than one, hence, under negative selection.