Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China

• DOI: 10.1007/s00705-014-2080-9
• Publication Date: 2014-04-16T04:11:13Z
• Authors: Hu Weiwei; Yu Qinghua; Zhu Liqi; Liu Haofei; Zhao Shanshan; Gao Qi; He Kongwang; Yang Qian
• Keywords: 0301 basic medicine; China; 0303 health sciences; Transmissible gastroenteritis virus; Sequence Homology; Genome, Viral; Sequence Analysis, DNA; 3. Good health; Viral Proteins; 03 medical and health sciences; Mutation; Animals; Cluster Analysis; RNA, Viral; Original Article; 14. Life underwater; Phylogeny

A strain of transmissible gastroenteritis virus (TGEV), SHXB, was isolated in Shanghai, China. The complete genome of strain SHXB was sequenced, and its sequence was compared those of other TGEV strains in the GenBank database. The comparison showed that there were no insertions or deletions in the 5' and 3'- non-translated regions, in the nonstructural genes ORF1, ORF3, and ORF7, or in the genes encoding the structural proteins envelope (E), membrane (M) and nucleoprotein (N). A phenomenon in common with other strains was that nucleotide (nt) 655 of the spike (S) gene was G, and a common change in nt 1753 of the S gene was a T-to-G mutation that caused a serine-to-alanine mutation at amino acid 585, which is in the region of the main major antigenic sites A and B of the TGEV S protein. A 6-nt deletion was also found at nt 1123-1128 in all Purdue strains except the strain Virulent Purdue. Phylogenetic analysis showed that TGEV SHXB was closely related to the Purdue strains and shared a common ancestor with the Miller strains as well as strain PRCV-ISU-1.