The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28

• DOI: 10.1007/s00705-014-2218-9
• Publication Date: 2014-09-23T23:39:48Z
• Authors: Yanhong Lin; Hailong Zhang; Chengjin Zhao; Shengxue Liu; Lihua Guo
• Keywords: 0303 health sciences; Base Sequence; Sequence Analysis, RNA; Alternaria; Genome, Viral; RNA-Dependent RNA Polymerase; 3. Good health; Open Reading Frames; Viral Proteins; 03 medical and health sciences; RNA Viruses; RNA, Viral; Viruses, Unclassified; Amino Acid Sequence; 5' Untranslated Regions; 3' Untranslated Regions; Phylogeny; RNA, Double-Stranded

The complete nucleotide sequence of Alternaria longipes dsRNA virus 1 (AlRV1), a novel double-stranded RNA (dsRNA) mycovirus, was determined and analyzed. AlRV1-HN28 contains a single dsRNA genome segment 3415 base pairs in length (excluding the 3' poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORFs, ORF A and ORF B). The 5'-proximal ORF A (1182 nt) potentially encodes a protein of 394 amino acids (aa) with a predicted molecular mass of 43 kDa; this protein showed no significant similarities to any other sequences in any of the NCBI protein databases. The 3'-proximal ORF B (1737 nt) encodes a protein of 579 aa with a predicted molecular mass of 65 kDa; this protein sequence shares similarities with the conserved domains of RNA-dependent RNA polymerases of other mycoviruses. Phylogenetic analysis indicated that AlRV1-HN28 was closely related to four other unclassified viruses, which suggests that the AlRV1-HN28 isolated from Alternaria longipes may belong to a new family of dsRNA mycoviruses. This is the first report of the full-length nucleotide sequence of a mycovirus that infects Alternaria longipes.