Molecular characterization and complete genome of a novel nepovirus from red clover

• DOI: 10.1007/s00705-018-3742-9
• Publication Date: 2018-02-03T03:31:03Z
• Authors: Igor Koloniuk; Jaroslava Přibylová; Jana Fránová
• Keywords: 0301 basic medicine; Viral Proteins; 0303 health sciences; 03 medical and health sciences; Nepovirus; High-Throughput Nucleotide Sequencing; RNA, Viral; Trifolium; Genome, Viral; Phylogeny; Plant Diseases; 3. Good health

During high throughput sequencing (HTS) of leaves from a symptomatic red clover plant, a new RNA virus, tentatively named red clover nepovirus A (RCNVA), was discovered. The complete genomic sequence was determined and characterized. Particularly noteworthy was that RCNVA shares high sequence identities in RNA1 with a group of phylogenetically related nepoviruses while homologies in the RNA2 segments are markedly lower. Based on the genomic organization and phylogenetic attributes, RCNVA should be classified as a novel virus of the genus Nepovirus (subfamily Comovirinae, family Secoviridae, order Picornavirales).