Transmission studies of the newly described apple chlorotic fruit spot viroid using a combined RT-qPCR and droplet digital PCR approach

• DOI: 10.1007/s00705-020-04704-5
• Publication Date: 2020-07-08T01:02:36Z
• Authors: Thomas Leichtfried; Helga Reisenzein; Siegrid Steinkellner; Richard A. Gottsb...
• Keywords: Myzus persicae

Abstract The transmission of the apscaviroid tentatively named apple chlorotic fruit spot viroid (ACFSVd) was investigated using a one-step reverse-transcription (RT) droplet digital PCR assay for absolute quantification viroid, followed by relative standard curves RT-qPCR. Our results indicate that ACFSVd is effectively transmitted grafting, budding and seeds. No has yet been observed to viroid-inoculated pome species Pyrus sp. Cydonia detected in viruliferous aphids ( Myzus persicae , Dysaphis plantaginea ) codling moths Cydia pomonella ). also systemically infected hemiparasitic plant Viscum album subsp. (mistletoe).