Identification and genome analysis of a tomato zonate spot virus isolate from Bidens pilosa

• DOI: 10.1007/s00705-021-05330-5
• Publication Date: 2022-01-11T00:03:06Z
• Authors: Tengzhi Xu; Lei Lei; Xiangru Chen; Rongyu Li; Xiaomao Wu; Youhua Long; Meng-ao Jia
• Keywords: 0301 basic medicine; 03 medical and health sciences; Solanum lycopersicum; Tospovirus; Bidens; Phylogeny; Plant Diseases

Bidens pilosa is a weed species that invades crop areas in tropical and subtropical regions. To date, only two potyviruses have been reported to infect B. pilosa. Here, we report the complete genome sequence of a tomato zonate spot tospovirus (TZSV) isolate from Bidens named TZSV-Bidens. The tripartite RNA of the TZSV-Bidens genome contains L, M, and S segments that are 8912, 4724, and 2997 nt in length, respectively. The genome contains five open reading frames (ORFs), with 92.23-95.01% amino acid sequence identity to the TZSV-YN isolate. Phylogenetic analysis based on amino acid sequences of members of the family Tospoviridae showed that TZSV-Bidens was grouped into a well-supported Eurasian cluster. The intergenic regions (IGRs) of the M and S RNAs are among the most variable regions and are far shorter than those of the TZSV-YN reference genome.