Human splanchnic amino-acid metabolism
CITRULLINE
Male
0301 basic medicine
DIET-INDUCED OBESITY
Colon
Clinical Biochemistry
Gut microbiota
Hepatic Veins
Kidney
Biochemistry
Renal Veins
Pancreaticoduodenectomy
03 medical and health sciences
Mesenteric Veins
Duodenal Neoplasms
Intestine, Small
Humans
Obesity
Splanchnic Circulation
Amino Acids
IN-VIVO
Aged
Interorgan metabolism
SMALL-INTESTINE
INSULIN-RESISTANCE
NITRIC-OXIDE
Portal Vein
GUT MICROBIOTA
Organic Chemistry
Type 2 diabetes
Middle Aged
GLUTAMINE
3. Good health
Pancreatic Neoplasms
Liver
Radial Artery
ARGININE METABOLISM
RENAL METABOLISM
Amino acids
Original Article
Female
Spleen
DOI:
10.1007/s00726-016-2344-7
Publication Date:
2016-10-06T01:53:26Z
AUTHORS (9)
ABSTRACT
Plasma levels of several amino acids are correlated with metabolic dysregulation in obesity and type 2 diabetes. To increase our understanding of human amino-acid metabolism, we aimed to determine splanchnic interorgan amino-acid handling. Twenty patients planned to undergo a pylorus preserving pancreatico-duodenectomy were included in this study. Blood was sampled from the portal vein, hepatic vein, superior mesenteric vein, inferior mesenteric vein, splenic vein, renal vein, and the radial artery during surgery. The difference between arterial and venous concentrations of 21 amino acids was determined using liquid chromatography as a measure of amino-acid metabolism across a given organ. Whereas glutamine was significantly taken up by the small intestine (121.0 ± 23.8 µmol/L; P < 0.0001), citrulline was released (-36.1 ± 4.6 µmol/L; P < 0.0001). This, however, was not seen for the colon. Interestingly, the liver showed a small, but a significant uptake of citrulline from the circulation (4.8 ± 1.6 µmol/L; P = 0.0138) next to many other amino acids. The kidneys showed a marked release of serine and alanine into the circulation (-58.0 ± 4.4 µmol/L and -61.8 ± 5.2 µmol/L, P < 0.0001), and a smaller, but statistically significant release of tyrosine (-12.0 ± 1.3 µmol/L, P < 0.0001). The spleen only released taurine (-9.6 ± 3.3 µmol/L; P = 0.0078). Simultaneous blood sampling in different veins provides unique qualitative and quantitative information on integrative amino-acid physiology, and reveals that the well-known intestinal glutamine-citrulline pathway appears to be functional in the small intestine but not in the colon.
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