Human splanchnic amino-acid metabolism

• DOI: 10.1007/s00726-016-2344-7
• Publication Date: 2016-10-06T01:53:26Z
• Authors: Evelien P. J. G. ...; S. Sabrkhany; I. Hundscheid; D. Schellekens; K. Lenaerts; S. W. Olde Damink; E. E. Blaak; C. H. C. Dejong; Sander S. Rensen
• Keywords: Splanchnic Circulation; Alanine

Plasma levels of several amino acids are correlated with metabolic dysregulation in obesity and type 2 diabetes. To increase our understanding human amino-acid metabolism, we aimed to determine splanchnic interorgan handling. Twenty patients planned undergo a pylorus preserving pancreatico-duodenectomy were included this study. Blood was sampled from the portal vein, hepatic superior mesenteric inferior splenic renal radial artery during surgery. The difference between arterial venous concentrations 21 determined using liquid chromatography as measure metabolism across given organ. Whereas glutamine significantly taken up by small intestine (121.0 ± 23.8 µmol/L; P < 0.0001), citrulline released (−36.1 4.6 0.0001). This, however, not seen for colon. Interestingly, liver showed small, but significant uptake circulation (4.8 1.6 = 0.0138) next many other acids. kidneys marked release serine alanine into (−58.0 4.4 µmol/L −61.8 5.2 µmol/L, smaller, statistically tyrosine (−12.0 1.3 spleen only taurine (−9.6 3.3 0.0078). Simultaneous blood sampling different veins provides unique qualitative quantitative information on integrative physiology, reveals that well-known intestinal glutamine–citrulline pathway appears be functional