SDF-1 mediates mesenchymal stem cell recruitment and migration via the SDF-1/CXCR4 axis in bone defect

• DOI: 10.1007/s00774-020-01122-0
• Publication Date: 2020-10-20T08:04:01Z
• Authors: Heli Zhang; Xijing Li; Junfeng Li; Lili Zhong; Xue Chen; Si Chen
• Keywords: Male; 0301 basic medicine; Receptors, CXCR4; 0303 health sciences; Bone Transplantation; Mesenchymal Stem Cells; Mesenchymal Stem Cell Transplantation; Bone and Bones; Chemokine CXCL12; Up-Regulation; Rats; Disease Models, Animal; 03 medical and health sciences; Bone Marrow; Cell Movement; Osteogenesis; Animals; Femur; RNA, Messenger; Rats, Wistar; Biomarkers; Signal Transduction

Recent studies have indicated the potential of stem cell therapy in combination with cytokines to restore the bone repair via migration and homing of stem cells to the defected area. The present study aimed to investigate the mobilization and recruitment of mesenchymal stem cells (MSCs) in response to SDF-1.Herein, the knockout rat model of the bone defect (BD) was treated with the induced membrane technique. Then, wild type Wistar rats and SDF-1-knockout rats were selected for the establishment of BD-induced membrane (BD-IM) models and bone-graft (BG) models. The number of MSCs was evaluated by flow cytometry, along with the expression pattern of the SDF-1/CXCR4 axis as well as osteogenic factors was identified by RT-qPCR and Western blot analyses. Finally, the MSC migration ability was assessed by the Transwell assay.Our data illustrated that in the induced membrane tissues, the number of MSCs among the BD-IM modeled rats was increased, whereas, a lower number was documented among BG modeled rats. Besides, we found that lentivirus-mediated over-expression of SDF-1 in BG modeled rats could activate the SDF-1/CXCR4 axis, mobilize MSCs into the defect area, and up-regulate the osteogenic proteins.Collectively, our study speculated that up-regulation of SDF-1 promotes the mobilization and migration of MSCs through the activation of the SDF-1/CXCR4 signal pathway.