Antimicrobial effects of clindamycin-loaded platelet-rich fibrin (PRF)

• DOI: 10.1007/s00784-024-05532-6
• Publication Date: 2024-02-13T20:02:06Z
• Authors: Anton Straub; Maximilian Stapf; Chiara Utz; Andreas Vollmer; Julia Flesch; Alexander Kübler; Oliver Scherf-Clavel; Thiên-Trí Lâm; Stefan Hartmann
• Keywords: Platelet-rich fibrin; Fusobacterium nucleatum; Agar diffusion test

Abstract Objectives Recent research has demonstrated that platelet-rich fibrin (PRF) is an appropriate carrier for ampicillin/sulbactam. The aim of the study was to investigate whether PRF also a suitable bio-carrier clindamycin (CLI). Methods membranes were produced from 36 patients receiving intravenous therapy with CLI (e.g. due diagnosis osteonecrosis jaw or infections). Concentrations in measured liquid chromatography-tandem mass spectrometry, and antimicrobial effects investigated vitro agar diffusion tests fresh stored 24 h. Storage performed incubator at °C simulate in-vivo situation. Results mean concentration plasma 1.0 ± 0.3 μg/100 mg plasma; resulting 0.7 0.4 PRF. Agar Staphylococcus aureus , Streptococcus pneumoniae mitis Porphyromonas gingivalis, Fusobacterium nucleatum . Mean inhibition zones, mm, 17.3, 12.2, 18.8, 17.1, 25.8 18.1, 12.7, 19.2, 26.3 PRF, respectively. Conclusion results demonstrate when administered systemically patients. generated after infusion 600 dose suffices target clinically relevant bacteria. Clinical relevance Using as local antibiotic application can prevent infections oral maxillofacial surgery. Within limitations, findings could expand scope by adding new spectrum therapy.