The monofunctional CO dehydrogenase CooS is essential for growth of Thermoanaerobacter kivui on carbon monoxide

• DOI: 10.1007/s00792-021-01251-y
• Publication Date: 2021-12-17T18:20:37Z
• Authors: Surbhi Jain; Alexander Katsyv; Mirko Basen; Volker Müller
• Keywords: Carbon monoxide dehydrogenase

Thermoanaerobacter kivui is a thermophilic acetogen that can grow on carbon monoxide as sole and energy source. To identify the gene(s) involved in CO oxidation, genome sequence was analyzed. Two genes potentially encoding dehydrogenases were identified. One, cooS, encodes monofunctional dehydrogenase, whereas another, acsA, CODH component of CODH/ACS complex. Both cloned, His-tag added, proteins produced from plasmid T. kivui. His-AcsA copurified by affinity chromatography with AcsB, acetyl-CoA synthase dehydrogenase/acetyl CoA His-CooS CooF