Detection of SYT-SSX Rearrangements in Synovial Sarcomas by Real-Time One-Step RT-PCR

• DOI: 10.1007/s10024-004-8097-4
• Publication Date: 2005-03-03T14:59:02Z
• Authors: Marina N. Nikiforova; Pamela Groen; George Mutema; Yuri E. Nikiforov; David Witte
• Keywords: Synovial Sarcoma

Synovial sarcomas are aggressive tumors of adolescent and young adults that account for up to 10% soft tissue sarcomas. Cytogenetically, they characterized by translocation t(X;18), which is found in more than 95% tumors. In most cases, it results fusion the SYT gene with SSX1 or SSX2 gene, thus creating SYT-SSX1 SYT-SSX2 rearrangement. The 2 types have been correlated histologic variants prognosis synovial this study, we developed a simple rapid method simultaneous detection rearrangements using LightCycler real-time one-step reverse transcriptase polymerase chain reaction (RT-PCR) technology (Roche). Oligonucleotide probes were designed so donor probe would span point acceptor be complementary sequence but nucleotide mismatches sequence. Such design allows amplification rearrangement same distinguishes them based on differences melting temperature detected curve analysis after PCR. With method, 27 (9 18 nonsynovial sarcomas) studied showed 6 cases 3 cases. These had complete correlation finding conventional RT-PCR direct sequencing. conclusion, fast, accurate, major SYT-SSX analysis.