Phase and fluorescence imaging by combination of digital holographic microscopy and fluorescence microscopy

• DOI: 10.1007/s10043-015-0042-4
• Publication Date: 2015-03-03T13:20:35Z
• Authors: Xiangyu Quan; Kouichi Nitta; Osamu Matoba; Peng Xia; Yasuhiro Awatsuji
• Keywords: Digital Holographic Microscopy; Digital holography; Fluorescence-lifetime imaging microscopy; Photoactivated localization microscopy; Digital Imaging

Hybrid digital holographic microscopy that combines fluorescence microscopy and digital holographic microscopy into a single system for biological applications is proposed. In the proposed system, a phase image and a fluorescence image can be obtained simultaneously by selecting the different wavelengths of the fluorescent light and the phase measurement. Especially for biological applications, the cell structure can be obtained by the phase imaging based on digital holography and nucleus of the cell can be obtained by the fluorescence imaging. The measurement of fluorescence beads and egera densa presented the feasibility of simultaneous detection of both a phase image and a fluorescence image.