Bronchial microbiome of severe COPD patients colonised by Pseudomonas aeruginosa

• DOI: 10.1007/s10096-013-2044-0
• Publication Date: 2014-01-21T07:14:46Z
• Authors: L. Millares; R. Ferrari; M. Gallego; M. Garcia-Nuñez; V. Pérez-Brocal; M. Espasa; X. Pomares; C. Monton; A. Moya; E. Monsó
• Keywords: Moraxella (Branhamella) catarrhalis; Colonisation; Fusobacteria

The bronchial microbiome in severe COPD during stability and exacerbation patients chronically colonised by Pseudomonas aeruginosa (PA), has not been defined. Our objective was to determine the characteristics of P. its changes exacerbation. with disease frequent exacerbations were categorised according chronic colonisation aeruginosa. Sputum samples obtained exacerbation, cultured, analysed 16S rRNA gene amplification pyrosequencing. Sixteen included, 5 them showing genus had significantly higher relative abundance stable (p = 0.019), but no significant differences biodiversity parameters found between two groups (Shannon, 3 (2-4) vs (2-3), p 0.699; Chao1, 124 (77-159) 140 (115-163), 0.364). In PA-colonised changed a similar non-PA-colonised exacerbations. An increase over 20 % for Streptococcus, Pseudomonas, Moraxella, Haemophilus, Neisseria, Achromobacter Corynebacterium genera, which include recognised potentially pathogenic microorganisms, 13 paired samples. These increases identified culture out participants (38.5 %). Stable showed patients, secretions. Exacerbation same microbial pattern, independently previous