Coexistence of tet(A) and blaKPC-2 in the ST11 hypervirulent tigecycline- and carbapenem-resistant Klebsiella pneumoniae isolated from a blood sample

• DOI: 10.1007/s10096-022-04512-6
• Publication Date: 2022-11-02T07:02:31Z
• Authors: Xiaokui Zhu; Changwu Yue; Huaixin Geng; Lingjie Song; Huiming Yuan; Xianqin Zhang; Chuanyu Sun; Guangxin Luan; Xu Jia
• Keywords: Tigecycline; Medical microbiology; Carbapenem

Carbapenem-resistant Klebsiella pneumoniae are distributed worldwide. This study aimed to characterize a hypervirulent tigecycline-resistant and carbapenem-resistant strain, XJ-K2, collected from patient's blood. We tested antimicrobial susceptibility, virulence, whole-genome sequencing (WGS) on strain XJ-K2. WGS data were used identify virulence resistance genes perform multilocus sequence typing (MLST) phylogenetic analysis. Three novel plasmids, including pLVPK-like plasmid (pXJ-K2-p1) two multiple plasmids (pXJ-K2-KPC-2 pXJ-K2-p3), discovered in The IncFII(pCRY) pXJ-K2-p3 carried the dfrA14, sul2, qnrS1, blaLAP-2, tet(A) genes. IncFII(pHN7A8)/IncR pXJ-K2-KPC-2 also range of elements, containing rmtB, blaKPC-2, blaTEM-1, blaCTX-M-65, fosA3. MLST analysis revealed that XJ-K2 belonged type 11 (ST11). Seven complete phage sequences many found Meanwhile, susceptibility tests G. mellonella larval infection models confirmed extensively drug (XDR) hypervirulence KJ-K2. To our knowledge, this is first observation description ST11 tigecycline- K. co-carrying blaKPC-2 blood China. Further investigation needed understand mechanisms significant strain.