Development of a colorimetric loop-mediated isothermal amplification assay for Helicobacter pylori detection

• DOI: 10.1007/s10096-025-05153-1
• Publication Date: 2025-05-14T02:31:49Z
• Authors: Chonticha Romyasamit; Apichat Kaewdech; Pimsiri Sripongpun; Naichaya Chamroonkul; Maseetoh Samaeng; Dechawat Wongprot; Phanvasri Saengsuwan; Morteza Saki; Phoomjai Sornsenee
• Keywords: Medical microbiology; Helicobacter Infections

Helicobacter pylori is an important pathogen responsible for various gastrointestinal disorders, including peptic ulcers and gastric cancer. Rapid accurate detection of H. infection crucial its early diagnosis treatment. This study aimed to develop evaluate the efficacy a colorimetric loop-mediated isothermal amplification (C-LAMP) assay in tissue biopsy samples. In total, 302 samples were collected, performance C-LAMP was compared with that conventional diagnostic methods, culture, PCR, rapid urease test (CLO test). The limit 1 CFU/mL reaction time 15 min at 61 °C, highlighting efficiency diagnosis. Compared demonstrated sensitivity 80% specificity 98%, whereas 60% 100%. ROC analysis revealed superior accuracy (AUC = 0.80 using PCR as reference; AUC 0.89 culture reference) relative CLO 0.63 vs. PCR; 0.65 culture), 0.60 PCR), 0.78 culture). These results suggest highly sensitive, specific, cost-effective tool pylori, offering significant advantages over particularly resource-limited settings, promising alternative reliable both clinical field settings.