Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts

• DOI: 10.1007/s10103-008-0582-y
• Publication Date: 2008-07-03T15:07:53Z
• Authors: Carla Andreotti D...; Giorgio De Micheli; Sueli Patrícia Ha...; Ilíria Salomão Feist; Márcia Martins Ma...
• Keywords: Gingivoplasty; Wound Healing; Fibroblast Growth Factor 7; Gingiva; Fibroblasts; Phototherapy; Culture Media; 3. Good health; Fibroblast Growth Factors; 03 medical and health sciences; 0302 clinical medicine; Animals; Humans; Cattle; Fibroblast Growth Factor 2; Low-Level Light Therapy; Cells, Cultured

The effects of laser phototherapy on the release of growth factors by human gingival fibroblasts were studied in vitro. Cells from a primary culture were irradiated twice (6 h interval), with continuous diode laser [gallium-aluminum-arsenium (GaAlAs), 780 nm, or indium-gallium-aluminum-phosphide (InGaAlP),_660 nm] in punctual and contact mode, 40 mW, spot size 0.042 cm(2), 3 J/cm(2) and 5 J/cm(2) (3 s and 5 s, respectively). Positive [10% fetal bovine serum (FBS)] and negative (1%FBS) controls were not irradiated. Production of keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) was quantified by enzyme-linked immunosorbent assay (ELISA). The data were statistically compared by analysis of variance (ANOVA) followed by Tukey's test (P </= 0.05). The characterization of the cell line indicated a mesenchymal nature. KGF release was similar in all groups, while that of bFGF was significantly greater (1.49-times) in groups treated with infra-red laser. It was concluded that increased production of bFGF could be one of the mechanisms by which infra-red laser stimulates wound healing.