Effects of photodynamic therapy on Enterococcus faecalis biofilms

• DOI: 10.1007/s10103-015-1749-y
• Publication Date: 2015-04-27T11:04:19Z
• Authors: L. López-Jiménez; E. Fusté; B. Martínez-Garriga; J. Arnabat-Domínguez; T. Vinuesa; M. Viñas
• Keywords: Enterococcus faecalis; Methylene blue

Microbial biofilms are involved in almost all infectious pathologies of the oral cavity. This has led to search for novel therapies specifically aimed at biofilm elimination. In this study, we used atomic force microscopy (AFM) visualize injuries and determine surface roughness, as well confocal laser scanning (CLSM) enumerate live dead bacterial cells, effects photodynamic therapy (PDT) on Enterococcus faecalis biofilms. The AFM images showed that PDT consisting methylene blue a 670-nm diode (output power 280 mW during 30 s) or toluidine 628-nm LED light 1000 induced severe damage, including cell lysis, E. biofilms, with former also causing an important increase roughness. These observations were confirmed by cells determined using CLSM. Our results highlight potential promising method achieve successful disinfection.