Differences in the intraspecies copy number variation of Arabidopsis thaliana conserved and nonconserved miRNA genes

• DOI: 10.1007/s10142-023-01043-x
• Publication Date: 2023-04-10T08:03:03Z
• Authors: Anna Samelak-Czajka; Pawel Wojciechowski; Malgorzata Marsza...; Marek Figlerowicz; Agnieszka Zmienko
• Keywords: Silique

MicroRNAs (miRNAs) regulate gene expression by RNA interference mechanism. In plants, miRNA genes (MIRs) which are grouped into conserved families, i.e. they present among the different plant taxa, involved in regulation of many developmental and physiological processes. The roles nonconserved MIRs-which MIRs restricted to one family, genus, or even species-are less recognized; however, them participate responses biotic abiotic stresses. Both over- underproduction miRNAs may influence various biological Consequently, maintaining intracellular homeostasis seems be crucial for organism. Deletions duplications genomic sequence alter dosage and/or activity. We evaluated extent copy number variations (CNVs) Arabidopsis thaliana (Arabidopsis) over 1000 natural accessions, using population-based analysis short-read sequencing data. showed that were unlikely display CNVs their deletions extremely rare, whereas presented moderate variation. Transposon-derived displayed exceptionally high diversity. Conversely, epigenetic control transposons reactivated during development mostly invariable. MIR overlap with protein-coding also limited variability. At level, a higher rate nonvariable, was detectable Col-0 leaves, inflorescence, siliques compared variable miRNAs, although both groups much lower than MIRs. Our data indicate CNV is related age, function, localization.