UDP-glycosyltransferase gene SlUGT73C1 from Solanum lycopersicum regulates salt and drought tolerance in Arabidopsis thaliana L.

• DOI: 10.1007/s10142-023-01242-6
• Publication Date: 2023-10-16T14:02:06Z
• Authors: Mengdi Lu; Jing Guo; Dingxiao Dong; Meiyu Zhang; Qian Li; Yaoliang Cao; Yanlong Dong; Chao Chen; Xiaoxia Jin
• Keywords: 0301 basic medicine; 0303 health sciences; Arabidopsis; Glycosyltransferases; 15. Life on land; Sodium Chloride; Plants, Genetically Modified; 6. Clean water; Uridine Diphosphate; Droughts; Solanum lycopersicum; Gene Expression Regulation, Plant; Stress, Physiological; Drought Resistance; Abscisic Acid; Plant Proteins

Among abiotic stresses, plants are the most vulnerable to salt and drought stresses. These stresses affect plant growth and development. Glycosyltransferases are involved in the responses of plants to abiotic stresses. In this study, a UDP-glycosyltransferase gene (SlUGT73C1) from Solanum lycopersicum was isolated and identified, which exhibited induction under salt or drought stress. The full length of SlUGT73C1 was 1485 bp, encoding 494 amino acids. Stress-related cis-acting elements were present in the promoter sequence of SlUGT73C1, such as ARE, LTR, and GC motifs. Compared with the wild-type plants, Arabidopsis thaliana overexpressing SlUGT73C1 exhibited increased seed germination rate and SOD and POD activities, decreased MDA content, and increased expression levels of osmotic stress regulators genes, rate-limiting enzymes genes in the proline synthesis pathway, Na+/K+ reverse transporter genes, and rate-limiting genes in the ABA biosynthesis pathway under salt or drought stress. These results indicated that SlUGT73C1 plays an important role in regulating salt and drought tolerance in plants.