Huang Qin decoction increases SLC6A4 expression and blocks the NFκB-mediated NLRP3/Caspase1/GSDMD pathway to disrupt colitis-associated carcinogenesis

• DOI: 10.1007/s10142-024-01334-x
• Publication Date: 2024-03-12T06:07:44Z
• Authors: Yili Tao; Lai Wang; Xiaofeng Ye; Xin Qian; Danye Pan; Xiaoyu Dong; Qian Jiang; Po Hu
• Keywords: Mesalazine; Proinflammatory cytokine

Abstract Huang Qin decoction (HQD) is a traditional Chinese medicine formula for treating colitis, but the effects and molecular mechanism of action HQD in colitis-associated carcinogenesis (CAC) are still unclear. Therefore, we aimed to determine beneficial on CAC mice reveal underlying involved. AOM/DSS was used induce mice, tumorigenesis were examined (with mesalazine serving as positive control). Mesalazine or treatment alleviated body weight loss decreased disease activity index induced by AOM/DSS. also suppressed shortening colon tissue length, number tumors, infiltration inflammatory cells. The genes targeted predicted verified, followed knockout experiments. Elevated SLC6A4 inhibited serotonin production inflammation observed HQD-treated mice. NFκB NLRP3/caspase1/GSDMD pathways. therapeutic effect diminished SLC6A4-deficient Additionally, downregulation mitigated inhibitory HQD-containing serum MODE-K cell pyroptosis. Our findings suggest that pivotal regulator HQD-alleviated via its modulation pathway.