Development of a rapid assay for screening point mutations associated with quinolone resistance in the Pseudomonas aeruginosa parC gene
DNA Topoisomerase IV
0301 basic medicine
4-Quinolones
In Vitro Techniques
Polymerase Chain Reaction
3. Good health
03 medical and health sciences
Anti-Infective Agents
Drug Resistance, Bacterial
Pseudomonas aeruginosa
Humans
Point Mutation
Genetic Testing
DNA Primers
DOI:
10.1007/s101560050045
Publication Date:
2002-08-25T05:57:49Z
AUTHORS (7)
ABSTRACT
To detect quinolone resistance-associated mutations within the Ser-80 and Glu-84 codons of the Pseudomonas aeruginosa parC gene, we developed a rapid and simple assay based on polymerase chain reaction (PCR) amplification of the region of the parC gene containing the mutation sites and digestion of the PCR products with a restriction enzyme. The mutations generating alterations at Ser-80 and Glu-84 were detected as restriction fragment length polymorphisms of the PCR products digested with HinfI. Among 22 clinical isolates tested by this assay, mutations at the Ser-80 and Glu-84 codons were detected in all 10 isolates in which the presence of the mutations had been confirmed previously by DNA sequencing. This rapid and simple assay could be a useful screening device for genetic alterations associated with resistance to quinolones in the P. aeruginosa parC gene.
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CITATIONS (1)
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