Comparative transcriptomic analysis reveals novel genes and regulatory mechanisms ofTetragenococcus halophilusin response to salt stress

• DOI: 10.1007/s10295-014-1579-0
• Publication Date: 2015-01-07T02:56:12Z
• Authors: Licui Liu; Lifang Si; Xin Meng; Lixin Luo
• Keywords: 0301 basic medicine; Salinity; Transcription, Genetic; Osmolar Concentration; Enterococcaceae; Gene Expression Regulation, Bacterial; Salt Tolerance; Sodium Chloride; Up-Regulation; 03 medical and health sciences; Protein Biosynthesis; ATP-Binding Cassette Transporters; Transcriptome; Heat-Shock Proteins

AbstractTetragenococcus halophilus, a moderately halophilic Gram-positive bacterium, was isolated from Chinese style soy sauce. This species is a valuable resource for investigating salt tolerance mechanisms and improving salinity resistance in microorganisms. RNA-seq was used to sequence T. halophilus samples treated with 0 M (T1), 1 M (T2), and 3.5 M NaCl (T3). Comparative transcriptomic analyses of the different treatments were performed using gene ontology and Kyoto encyclopedia of genes and genome. The comparison of T1 and T2 by RNA-seq revealed that genes involved in transcription, translation, membrane system, and division were highly up-regulated under optimum salt condition. The comparison of T2 and T3 showed that genes related to heat shock proteins or the ATP-binding cassette transport systems were significantly up-regulated under maximum-salt condition. In addition, a considerable proportion of the significantly differently expressed genes identified in this study are novel. These data provide a crucial resource that may determine specific responses to salt stress in T. halophilus.