Micropropagation of red sanders (Pterocarpus santalinusL.) using mature nodal explants

• DOI: 10.1007/s10310-006-0230-y
• Publication Date: 2006-09-29T03:41:20Z
• Authors: Ekambaranellore P...; Patan Shaik Sha V...; Thoguru Jonh Vive...; Elu Singh Meru
• Keywords: Subculture (biology); Sprouting

In vitro clonal multiplication of Pterocarpus santalinus L. was achieved using mature nodal explants a 10-year-old elite quality tree. Combinations serial transfer technique and incorporation antioxidants (250mg/l L-ascorbic acid 50mg/l citric acid) into the culture medium helped to minimize browning improve explant survival during shoot sprouting. About 70% were sprouted on Murashige Skoog (MS) liquid containing 4.4μM 6-benzyladenine (BA). The harvest period also influenced bud break sprouting in explants. combination BA 2.2μM thidiazuron (TDZ) found be most suitable growth regulator for obtaining highest percentage segment (74%–75%), number secondary shoots per primary (two or three), length (5–6cm), new segments generated active (four five), coefficient (3.5) within 6 weeks. Repeated subculturing obtained from cultures enabled continuous production healthy axillary shoots. At end sixth passage, about 90% produced five six green shoots, each being 6.6cm long with seven nodes. Multiplication increased first subculture (5.4) (8.3). best rooting response solidified half-strength MS supplemented 4.9μM indole-3-butyric (IBA). micropropagated plantlets established successfully 20-cm pots mixture soil farmyard manure (4:1 ratio) formed leaflets.