Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici, and its races

• DOI: 10.1007/s10327-010-0224-7
• Publication Date: 2010-03-12T18:06:17Z
• Authors: Keigo Inami; Chizu Yoshioka; Yasushi Hirano; Masato Kawabe; Seiya Tsushima; Tohru Teraoka; Tsutomu Arie
• Keywords: Intergenic region; Primer (cosmetics); genomic DNA

Five primer/probe sets to identify the tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL), and its three races selectively were designed based on the rDNA-intergenic spacer and avirulence genes. Real-time PCR using genomic DNA from mycelia and soil DNA with the primer/probe sets allowed the successful identification of FOL and its races.