Decreasing activity of NF-κB inhibits the proliferation of A549 cells via the down-regulation of cyclin D1 expression

• DOI: 10.1007/s10330-007-0192-3
• Publication Date: 2008-06-02T16:53:03Z
• Authors: Jian Zhang; Yongjian Xu; Weining Xiong; Zhenxiang Zhang; Chunling Du; Lifen Qiao; Wang Ni; Shixin Chen
• Keywords: Cyclin D; Cyclin B; Cyclin A

To investigate the effect of decreasing activity of NF-κB on proliferation of A549 cell line and the possible molecular mechanism. The recombinant plasmid PCDNA3.1(+)/IκBα expressing IκBα was constructed. The recombinant plasmid was then transfected to A549 cell. The activity of NF-κB, cell proliferation, and cyclin D1 expression were observed. Our results showed that transfecting PCDNA3.1(+)/IκBα inhibited activity of NF-κB in A549 cells, and decreasing activity of NF-κB inhibited proliferation of A549 cells. Decreasing activity of NF-κB was accompanied with down-regulation of cyclin D1 expression. Decreasing activity of NF-κB inhibited proliferation of A549 cells, and the molecular mechanism of the inhibition effect may be down-regulation of cyclin D1 expression.