Effects of fixatives and storage duration on avian sperm morphology

• DOI: 10.1007/s10336-022-02015-x
• Publication Date: 2022-09-08T10:02:57Z
• Authors: Gaute Grønstøl; Marie Danielsen; Emily R. A. Cramer; Lars Erik Johanne...; Arild Johnsen; Emma Whittington; Jan T. Lifjeld
• Keywords: Fixative

Abstract Sperm cells are variable both within and among species. To be able to accurately measure sperm understand their function, it is important that preserved in a manner maintains structural integrity. Formalin widely used fixative storage medium for cells, but few studies have examined the effect of fixation long-term on morphological Ethanol also common agent tissue samples, here we examine if formalin or ethanol alters cell size We found no significant effects process fresh fixed ethanol. Further, there were consistent length changes stored over period 227 days, three years. A comparison across 13–14 years time showed small reduction 0.93%. Furthermore, initially remained quite stable dry glass slides minimum six months (we mean 0.18% after 6 months). The proportion with head damage was, however, much higher samples than those formalin. Overall, 70% had acrosome versus only 3% Finding intact measuring length, therefore, required greater effort samples. Our findings indicate use from study morphometrics justified either fixative, although clearly preserves better.