Acinetobacter stercoris sp. nov. isolated from output source of a mesophilic german biogas plant with anaerobic operating conditions

• DOI: 10.1007/s10482-021-01517-7
• Publication Date: 2021-02-19T01:50:35Z
• Authors: Dipen Pulami; Thorsten Schauss; Tobias Eisenberg; Jochen Blom; Oliver Schwengers; Jennifer K. Bender; Gottfried Wilharm; Peter Kämpfer; Stefanie P. Glaeser
• Keywords: DNA, Bacterial; 0301 basic medicine; 610; 7. Clean energy; 03 medical and health sciences; RNA, Ribosomal, 16S; ddc:610; Anaerobiosis; 16S rRNA; Phylogeny; ddc:610; Original Paper; Base Composition; Genome; Acinetobacter; Fatty Acids; ddc:630; Nucleic Acid Hybridization; Sequence Analysis, DNA; Bacterial Typing Techniques; Acinetobacter stercoris; Biofuels; 610 Medizin und Gesundheit; Biogas plant digestate

AbstractThe Gram-stain-negative, oxidase negative, catalase positive strain KPC-SM-21T, isolated from a digestate of a storage tank of a mesophilic German biogas plant, was investigated by a polyphasic taxonomic approach. Phylogenetic identification based on the nearly full-length 16S rRNA gene revealed highest gene sequence similarity toAcinetobacter baumanniiATCC 19606T(97.0%). Phylogenetic trees calculated based on partial rpoBandgyrBgene sequences showed a distinct clustering of strain KPC-SM-21TwithAcinetobacter gerneriDSM 14967T = CIP 107464Tand not withA. baumannii, which was also supported in the five housekeeping genes multilocus sequence analysis based phylogeny. Average nucleotide identity values between whole genome sequences of strain KPC-SM-21Tand next related type strains supported the novel species status. The DNA G + C content of strain KPC-SM-21Twas 37.7 mol%. Whole-cell MALDI-TOF MS analysis supported the distinctness of the strain to type strains of next relatedAcinetobacterspecies. Predominant fatty acids were C18:1ω9c (44.2%), C16:0(21.7%) and a summed feature comprising C16:1ω7c and/or iso-C15:02-OH (15.3%). Based on the obtained genotypic, phenotypic and chemotaxonomic data we concluded that strain KPC-SM-21Trepresents a novel species of the genusAcinetobacter, for which the nameAcinetobacter stercorissp. nov. is proposed. The type strain is KPC-SM-21T(= DSM 102168T = LMG 29413T).