Lysophosphatidic acid enhances human umbilical cord mesenchymal stem cell viability without differentiation via LPA receptor mediating manner

• DOI: 10.1007/s10495-017-1399-6
• Publication Date: 2017-08-01T09:54:56Z
• Authors: Narengerile Li; Ya-Li Yan; Sachaofu Fu; Rui-Juan Li; Peng-Fei Zhao; Xi-Yuan Xu; Jing-Ping Yang; Alatangaole Damirin
• Keywords: CD90

Human umbilical cord mesenchymal stem cells (hUC-MSCs) are potential stromal which regarded as the most feasible cell group in therapy. The maintenance of survival without differentiation is important transplantation and However, negative factors exist transplantation. Lysophosphatidic acid (LPA) a non-antigenic small molecule phospholipid induced several fundamental cellular responses, such proliferation, apoptosis migration. In this study we aimed to explore effects LPA on MSCs its availability We found that stimulated hUC-MSC proliferation protected hUC-MSCs from lipopolysaccharide (LPS) apoptosis. also observed CD29, CD44, CD73, CD90 CD105 were expressed, whereas CD34 CD45 not expressed hUC-MSCs, these makers have no change containing medium, indicated accelerated an undifferentiating status. demonstrated higher LPAR1 involved action. was associated with mediated Gi/o-proteins/ERK1/2 pathway. On other hand, LPS-induced through suppressing caspase-3 activation by coupled G protein, but Gi/o or Gq/11 hUC-MSC. Collectively, increased manner. Our findings provide anti-apoptotic agent having application prospect