Prevalence of white spot syndrome virus infection detected by one-step and nested PCR in selected tiger shrimp (Penaeus monodon) hatcheries

• DOI: 10.1007/s10499-007-9153-7
• Publication Date: 2007-12-27T12:39:10Z
• Authors: Farhana Ayub; Md. Yusuf Sarker; Md. Samsul Alam
• Keywords: White spot syndrome; Penaeus monodon; Primer (cosmetics); Tiger

White spot syndrome (WSS) is considered as a great threat to commercial farming of the tiger shrimp (Penaeus monodon). The causal agent WSS DNA virus called white (WSSV). prevalence this dreadful infection has been studied in five randomly selected hatcheries located Cox's Bazar district Bangladesh. Both one-step and nested polymerase chain reaction (PCR) involving two pairs primers, namely, 146F1/146R1 146F2/146R2, amplifying 1447 bp 941 fragments, respectively, were conducted detect WSSV. Out 60 collected shrimps, 12 (20%) found be positive by PCR, while 18 (30%) PCR. PCR was much more sensitive than specimens showing clinical signs for WSSV both Some apparently healthy samples also Among primer-pairs, inner pair fragment outer primer when used