Validation of 12 species-specific, tetrasomic microsatellite loci from the Russian sturgeon, Acipenser gueldenstaedtii, for genetic broodstock management

• DOI: 10.1007/s10499-018-0290-y
• Publication Date: 2018-08-02T05:37:39Z
• Authors: K. Kohlmann; P. Kersten; J. Geßner; O. Eroglu; S. Firidin; M. Ciorpac; E. Taflan; R. Suciu
• Keywords: Broodstock; Hatchery; Genetic Variability

The Russian sturgeon, Acipenser gueldenstaedtii, is a critically endangered fish species. Hatcheries are operated in several countries within its natural range to produce stocking material for release into the wild and also aquaculture purposes (caviar meat production). An appropriate genetic broodstock management (plan or strategy) required avoid negative effects, e.g., admixture hybridization of genetically differing stocks loss variability due inbreeding drift. Therefore, 11 tetrasomic microsatellite loci were newly isolated from sturgeon genome arranged together with an already known locus four multiplex PCR sets. These microsatellites used characterize three groups hatchery juveniles Germany (aquaculture production), Turkey, Romania (production material) as well group wild-caught adults Danube River, Romania. Based on groups, measured by mean number alleles per expected heterozygosity, differentiation between Nei's GST distance D, ability 12 detect unwanted reductions differentiate could be demonstrated. This set can identify those pairs spawners that transmit highest possible next generation.