Morphofunctional Properties of Human Platelets Treated with Silver Nanoparticles

• DOI: 10.1007/s10517-017-3966-9
• Publication Date: 2017-11-25T02:39:29Z
• Authors: M. S. Makarov; N. V. Borovkova; M. V. Storozheva
• Keywords: Blood Platelets; 0303 health sciences; Silver; Dose-Response Relationship, Drug; Primary Cell Culture; Metal Nanoparticles; Cytoplasmic Granules; Platelet Activation; Cell Degranulation; 03 medical and health sciences; Platelet Adhesiveness; Humans; Pseudopodia

We studied structural and functional properties of human platelets in the presence of nanosilver particles. Incubation with 0.05-5 μM silver nanoparticles suppressed platelet adhesion in a dose-dependent manner without affecting internal platelet structure; during adhesion, some granules were not exocytized. Spontaneous platelet activation was observed at nanoparticle concentrations 15-100 μM. Addition of 1-5 μM nanosilver to cells undergoing adhesion blocked massive platelet degranulation, but did not prevent the formation of lamellopodia. The maximum number of preserved granules in platelets was revealed in the presence of 2.5-5 μM silver nanoparticles: 50% after platelet preincubation with silver nanoparticles and 75-77% after stabilization of adherent platelets with silver nanoparticles.