Production of High-content Galacto-oligosaccharide by Enzyme Catalysis and Fermentation with Kluyveromyces marxianus

• DOI: 10.1007/s10529-006-9002-1
• Publication Date: 2006-06-09T09:09:44Z
• Authors: Chao-Chun Cheng; Mei-Ching Yu; Tzu-Chien Cheng; Dey-Chyi Sheu; Kow-Jen Duan; Wei-Lun Tai
• Keywords: 0106 biological sciences; Probiotics; Galactose; Oligosaccharides; 04 agricultural and veterinary sciences; Disaccharides; beta-Galactosidase; 01 natural sciences; Catalysis; Glucose Oxidase; Kluyveromyces; Bioreactors; 0404 agricultural biotechnology; Fermentation

Of three beta-galactosidases from Aspergillus oryzae, Kluyveromyces lactis and Bacillus sp., used for the production of low-content galacto- oligosaccharides (GOS) from lactose, the latter produced the highest yield of trisaccharides and tetrasaccharides. GOS production was enhanced by mixing beta-galactosidase glucose oxidase. The low-content GOS syrups, produced either by beta-galactosidase alone or by the mixed enzyme system, were subjected to the fermentation by Kluyveromyces marxianus, whereby glucose, galactose, lactose and other disaccharides were depleted, resulting in up to 97% and 98% on a dry weight basis of high-content GOS with the yields of 31% and 32%, respectively.