Recombinant expression of mouse osteocalcin protein in Escherichia coli

• DOI: 10.1007/s10529-007-9437-z
• Publication Date: 2007-06-28T15:26:02Z
• Authors: Ji-Hyun Kim; Soonok Park; Hae-Won Kim; Jun-Hyeog Jang
• Keywords: 0301 basic medicine; Osteoblasts; Osteocalcin; Alkaline Phosphatase; Recombinant Proteins; 3. Good health; Mice; 03 medical and health sciences; Durapatite; Cell Adhesion; Escherichia coli; Animals; Cells, Cultured; Protein Binding

Osteocalcin is the most abundant non-collagenous protein of bone. Recombinant mouse osteocalcin protein (mOC) that includes the highly conserved central domain for binding to hydroxyapatite (HA), a mineral component of bone, was expressed in Escherichia coli. Purified mOC protein exhibited a significant increase in HA adhesion and differentiation in osteoblast cells as well as binding to HA with high affinity.