Identification and functional analysis of BABY BOOM genes from Rosa canina

• DOI: 10.1007/s10535-014-0420-y
• Publication Date: 2014-04-30T21:15:05Z
• Authors: H. F. Yang; Y. P. Kou; B. Gao; T. M. A. Soliman; K. D. Xu; N. Ma; X. Cao; L. J. Zhao
• Keywords: Medicago truncatula

BABY BOOM (BBM), initially identified in Brassica napus, can enhance the shoot regeneration capacity tissue culture and is involved conversion from vegetative to embryogenic state. This study aimed isolate BBM orthologue genes Rosa canina analyse their functions. Two full-length cDNAs, designated RcBBM1 RcBBM2, were isolated R. by rapid amplification of cDNA ends (RACE). The predicted amino acid sequences two RcBBMs contained bbm-1 motif motifs typically conserved eudicotANT (euANT) lineage. Phylogenetic tree analysis showed that most closely related Glycine max Medicago truncatula. transcripts detected young roots, calluses, protocorm-like bodies (PLBs), whereas they undetectable stems, leaves, flowers. RcBBM1-GFP RcBBM2-GFP fusion proteins both localized nucleus. 35S::RcBBM1 35S::RcBBM2 transgenic Arabidopsis thaliana lines exhibited enhanced culture, but did not undergo spontaneous somatic embryogenesis. results suggest may be candidate for improving efficiency canina.