A protocol for isolation and identification and comparative characterization of primary osteoblasts from mouse and rat calvaria

• DOI: 10.1007/s10561-019-09751-0
• Publication Date: 2019-03-18T06:02:52Z
• Authors: Baoyan Liu; Yanqin Lu; Yong Wang; Luna Ge; Naixiang Zhai; Jinxiang Han
• Keywords: Calvaria; Isolation

Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily contaminated by other cells such as fibroblasts. Here, we established a protocol isolation purification of primary osteoblast enzyme sequential digestion differential adhesion. In addition, compared the phenotypic functional traits which are commonly employed in studies. The method applied equally to was corroborated its feasibility validity. results also provided us evidences experiments choosing certain time point give intervention do relevant tests.