Transforming growth factor-β1 treatment of oral cancer induces epithelial-mesenchymal transition and promotes bone invasion via enhanced activity of osteoclasts

• DOI: 10.1007/s10585-013-9570-0
• Publication Date: 2013-02-01T18:09:56Z
• Authors: Jingjing Quan; Moustafa Elhousiny; Newell W. Johnson; Jin Gao
• Keywords: Gentamicin protection assay

This study investigates relationships between EMT and bone invasion by OSCC. Three OSCC cell lines, SCC25, HN5, Tca8113 were artificially induced to display adding 5 ng/mL of TGF-β1 culture media for 1-3 days. Cell morphology phenotypic changes was examined immunocytochemical staining CK VIM. markers, cell-invasion factors, osteoclast-related molecules analysed at mRNA, gelatine protein levels real-time PCR, zymography Western blotting respectively. Mature osteoclasts differentiated from Raw264.7 cells treated conditioned medium (CM) with/without TGF-β1. Immunohistochemistry performed validate proteins CK, VIM, E-cad Snail1 in tissue samples with invasion. Results showed minimal VIM found SCC25 while stained strongly. markers Twist1 N-cad up-regulated; down-regulated all cells. Of factors associated invasion, MMP-2 unchanged MMP-9 increased Tca8113, HN5. For molecules, both MT1-MMP RANKL up-regulated, OPG CM pre-treated prolong survival up 4 All target validated These findings suggest that not only induces increase the capacity but also promotes which osteoclast survival. may enhance ability MMP2/9 resorbing favouring cancer