Isolation and characterization of microsatellite markers for Brachiaria brizantha (Hochst. ex A. Rich.) Stap

• DOI: 10.1007/s10592-009-9839-7
• Publication Date: 2009-04-11T16:58:02Z
• Authors: L. Jungmann; A. C. B. Sousa; J. Paiva; P. M. Francisco; B. B. Z. Vigna; C. B. do Valle; M. I. Zucchi; A. P. de Souza
• Keywords: 0301 basic medicine; 03 medical and health sciences

The first set of nuclear simple sequence repeat (SSR) loci for Brachiaria brizantha (Hochst. ex A. Rich.) Stap is described. A microsatellite-enriched library was constructed and 19 loci were characterized. About 13 SSR loci were found to be polymorphic and across-taxa amplification tests showed that six of them can be transferred to four other species of Brachiaria. This new SSR resource will be a powerful tool for population genetic studies of B. brizantha, for interspecific genetic studies within the genus Brachiaria, for mapping and for marker assisted selection in breeding.