Inhibitory Effects of Sildenafil on Small Intestinal Motility and Myoelectrical Activity in Dogs

• DOI: 10.1007/s10620-006-3190-3
• Publication Date: 2006-04-13T20:45:15Z
• Authors: Xiaohong Xu; J. D. Z. Chen
• Keywords: Myoelectric Complex, Migrating; Sensitivity and Specificity; Piperazines; Sildenafil Citrate; Disease Models, Animal; Random Allocation; 03 medical and health sciences; Dogs; 0302 clinical medicine; Purines; Reference Values; Area Under Curve; Intestine, Small; Animals; Female; Sulfones; Gastrointestinal Motility; Probability

Previous studies have shown that sildenafil inhibits the esophageal motility in both humans and animals. The aim of this study was to investigate the effects of sildenafil on intestinal myoelectrical activity and motility. The study was composed of 2 experiments and performed in 7 healthy female dogs with a duodenal cannula 20 cm beyond pylorus (19-26 kg). The first experiment was designed to study the effects of sildenafil on intestinal myoelectrical activity and it included 2 sessions each consisting of 30-minute baseline, 15-minute posttreatment (placebo or 100 mg sildenafil) and 90 minutes after a liquid meal. Intestinal myoelectrical activity was recorded during the entire experiment period. The second experiment was aimed to investigate the effect of sildenafil on intestinal motility and was performed immediately after a solid meal. Intestinal motility was measured by a manometric catheter inserted into the small intestine via the duodenum cannula for 30 minutes at baseline and 60 minutes after sildenafil. Sildenafil significantly reduced the amplitude but had no effect on the frequency and regularity of the intestinal myoelectrical activity. Sildenafil significantly inhibited postprandial intestinal contractions. Although the frequency of the contractions was not altered, the mean area under the curve was significantly reduced during the first 30 minutes (P < .03) and second 30 minutes after sildenafil (P < .03); the power of intestinal contractile activities was also significantly reduced during the first 30 minutes (P < .0004) and second 30 minutes after sildenafil (P < .0003) in comparison with baseline. In conclusion, sildenafil inhibits the amplitude of both intestinal contractile activity and intestinal slow waves.